สืบค้นงานวิจัย
Induction of defensin response to dengue infection in Aedes aegypti
Wasinpiyamongkol L. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Induction of defensin response to dengue infection in Aedes aegypti
ผู้แต่ง / หัวหน้าโครงการ (EN): Wasinpiyamongkol L.
บทคัดย่อ (EN): Innate immune-related defensin peptide expression, the major antimicrobial peptide (AMP) in the dengue vector mosquito Aedes aegypti, was analyzed following infection by dengue virus type 2 (strain 16681) (DENV-2). In vitro, the mosquito cell line C6/36 was exposed to dengue virus at different multiplicities of infection (MOI) in an hour; it was found that the expression level of defensin transcripts was dependent upon viral dose. In addition, using both polymerase chain reaction (PCR) and Western blotting assays to determine defensin transcription and translation at a series of times post-inoculation, we found that the time course of defensin transcripts correlated with the expression of defensin peptide post-infection. In vitro, female Ae. aegypti mosquitoes were fed different meals (DENV-2 infected blood, non-infectious blood and sugar) at 14 days post-feeding, to determine defensin transcription in response to dengue infection, by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) assay. The results showed that a peak of 4.25kDa peptide, composed of defensin, was predominantly induced by DENV-2 infected blood-fed mosquitoes. We observed that DENV-2 could trigger the defensin peptide, defined molecularly as an innate immune response. Therefore, this peptide may be involved in dengue infection and/or transmission. Improved understanding of the mosquito's responses to dengue virus should strengthen our understanding of this vector's innate immune system. © 2014 The Entomological Society of Japan.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925942709&doi=10.1111%2fens.12108&partnerID=40&md5=c8e646235e581e362cfacb8041a94d16
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Dengue virus type 2 (strain 16681)
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Wasinpiyamongkol L.. "Induction of defensin response to dengue infection in Aedes aegypti". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Induction of defensin response to dengue infection in Aedes aegypti
Wasinpiyamongkol L.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Relationships between dengue virus infection in mosquito vector, (Aedes aegypti), dengue cases and weather conditions in Samut Sakhon Province, Thailand Bioefficiency test of aerosol insecticide products against a dengue vector, Aedes aegypti Morbidity Rate Prediction of Dengue Hemorrhagic Fever (DHF) Using the Support Vector Machine and the Aedes aegypti Infection Rate in Similar Climates and Geographical Areas Efficacy Evaluation of Deltamethrin and Cypermethrin Against Insecticide-resistant and -susceptible Strains of Dengue Vector Aedes aegypti Evaluation of herbal aerosol spray against insecticide-resistant strains of Aedes aegypti, vector of dengue and Zika viruses Vertical transmission of Indian Ocean Lineage of chikungunya virus in Aedes aegypti and Aedes albopictus mosquitoes Laboratory and field evaluation on the efficacy of temephos for larval control of insecticide resistant strains of the dengue vector Aedes aegypti ..................................................... Chemical composition and mosquitocidal activity of Cymbopogon citratus essential oil against Aedes aegypti from dengue and chikungunya risk areas Field evaluation of Le-mos aerosol spray against insecticide-resistant Aedes aegypti mosquitoes, vector of dengue and Zika viruses Efficacy of essential oils from Thai medicinal plants against insecticide-resistant Aedes aegypti mosquito in dengue-risk areas in Thailand
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