สืบค้นงานวิจัย
Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation.
S. Chatsapsin - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation.
ผู้แต่ง / หัวหน้าโครงการ (EN): S. Chatsapsin
บทคัดย่อ (EN): The rubber tree latex yield can be improved through bark stimulation with Ethylene. Ethrel®, an ethylene releaser, induces marked changes in the physiology and metabolism of the latex cells. In this study, the Suppression Subtractive Hybridization (SSH) technique was performed to compare the Hevea latex transcriptomes from control and Ethrel stimulated trees. Preliminary bioinformatic analysis of our 2 latex SSH libraries (Stimulated vs Control and Control vs Stimulated) showed that among the # 2,200 total sequenced ESTs, 3 isoforms of Abscisic acid (ABA) Stress Ripening gene (ASR) were found : 2 ASR1 isoforms and one ASR2. One ASR1 (ASR1-1) was present in both control and stimulated tree enriched latex SSH libraries, while the other (ASR1-2) was present in the stimulated enriched latex library only. In contrast ASR2 was found in the control tree enriched SSH library only. The ASRs are plant-specific stress-regulated gene family with DNA-binding property, whose biological role remains uncertain. In tomato, ASR2 encodes a putative transcription factor likely to be involved in one of the signaling pathways of ABA. The other two members, ASR1 and ASR3 share high sequence identity with ASR2, in which ASR1 has a zinc dependent DNA-binding activity. To investigate the possible role of ASR genes in relation with rubber yield and ethylene stimulation, differential expression of these isoforms of ASRs were studied by semiquantitative RT-PCR. Preliminary results showed that the bulk of ASR1 isoforms were constitutively expressed, at a similar level, in both latex and inner bark, and not regulated by ethylene, while a specific isoform (ASR1-2) was expressed in the latex only, and not or late slightly down-regulated by ethylene. Our ASR2 isoform was latex specific, being constitutively expressed and significantly down regulated by ethylene in the latex only, but neither expressed nor regulated by ethylene in the inner bark. Based on recent reports on ASR proteins function, we propose a new hypothesis for the ASRs function in the latex cells.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): SSH library
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

S. Chatsapsin. "Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation.". ไม่ระบุหน่วยงาน. ม.ป.ป.

S. Chatsapsin. "Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation.". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation.
S. Chatsapsin
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Effects of drought and tapping for latex production on water relations of Hevea brasiliensis trees DIVERSITY OF THE CASSIICOLIN GENE IN CORYNESPORA CASSIICOLA OF RUBBER TREE IN CHINA Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree Differential expression of mitochondrial pyruvate dehydrogenase gene correlates with latex yield and tapping in rubber tree DEVELOPMENT AND CHARACTERIZATION OF EST-SSR MARKERS FROM DE NOVO TRANSCRIPTOME SEQUENCING DATA IN RUBBER TREE (HEVEA BRASILIENSIS) Effect of fertilization on latex yield potential and physiological parameters of clone PB 217 in Cambodia Lipid composition of Hevea brasiliensis latex and dry rubber: characterization and relation with some physical properties An insight into laticifers sucrose transporters regulation in relation with the Ethrel-stimulation of latex yield.
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