สืบค้นงานวิจัย
De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis
Mohamed Sathik M.B. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Mohamed Sathik M.B.
บทคัดย่อ (EN): In order to extend the cultivation of Hevea to non-traditional rubber growing regions, clones with improved tolerance to extreme agro-climatic conditions like drought and cold with productivity on par with the traditional regions need to be developed. For this purpose, genes/factors contributing for the abiotic stress tolerance have to be identified and incorporated in the molecular breeding programmes. Though various molecular level studies in Hevea have thrown lights on the stress responsive transcripts, a comprehensive report of stress responsive ESTs in not available. Hence, this study eas initiated with an objective to identify drought, low temperature and ethylene stress responsive genes/factors through new generation sequencing technology. cDNA library of drought and cold stressed samples (leaves from clone RRIM600) and ethephon treated latex samples (clone RRII 105) were sequenced (single/pair end) on an Illumina Hi-Seq platform followed by de novo assembly with Velvet and Oases softwares. While the minimum read length obtained was 200 bp, the maximum read length was 12673 with Oases. After quality check and filtrations, about 87-91% of the reads wee used for the assembly. The sequences were annotated against Arabidopsis, Populus, Ricinus and Hevea and the fold change in their expression level was worked out. While a total of 268 transcripts got up-regulated in the drought samples, 57 and 12 transcripts were unique to control and droughted samples, respectively. In cold treated samples, 960 transcripts got up-regulated and 50 and 216 transcripts were found unique to control and cold treated samples respectively. In the case of ethylene treated samples, while 49 genes got down-regulated, 175 genes were up-regulated and among them 24 transcripts were unique to ethephon treated samples. The details of the findings are discussed.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): abiotic stress responsive transcripts
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Mohamed Sathik M.B.. "De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Mohamed Sathik M.B.. "De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis
Mohamed Sathik M.B.
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) Lipid composition of Hevea brasiliensis latex and dry rubber: characterization and relation with some physical properties Cultural and Morphological Characterizations of Fusicoccum sp., the causal agent of rubber (Hevea brasiliensis) leaf blight in Malaysia Effectiveness of activated biochar from rubber (Hevea brasiliensis) seed shells and epicarp as an amelioration material against pesticide residues in soil Plant Hormones and Oxidative Stress in Hevea brasiliensis Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis Multiple Protective roles of the Berberine Bridge Enzyme Gene HbBBE1 in Hevea brasiliensis Muell. Arg. CURRENT CHALLENGESS FOR DE NOVO HEVEA BRASILIENSIS GENOME SEQUENCING Cloning and expression of HbACO2 of Hevea brasiliensis
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