สืบค้นงานวิจัย
B cell responses during secondary dengue virus infection are dominated by highly cross-reactive, memory-derived plasmablasts
Priyamvada L. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): B cell responses during secondary dengue virus infection are dominated by highly cross-reactive, memory-derived plasmablasts
ผู้แต่ง / หัวหน้าโครงการ (EN): Priyamvada L.
บทคัดย่อ (EN): Dengue virus (DENV) infection results in the production of both type-specific and cross-neutralizing antibodies. While immunity to the infecting serotype is long-lived, heterotypic immunity wanes a few months after infection. Epidemiological studies link secondary heterotypic infections with more severe symptoms, and cross-reactive, poorly neutralizing antibodies have been implicated in this increased disease severity. To understand the cellular and functional properties of the acute dengue virus B cell response and its role in protection and immunopathology, we characterized the plasmablast response in four secondary DENV type 2 (DENV2) patients. Dengue plasmablasts had high degrees of somatic hypermutation, with a clear preference for replacement mutations. Clonal expansions were also present in each donor, strongly supporting a memory origin for these acutely induced cells. We generated 53 monoclonal antibodies (MAbs) from sorted patient plasmablasts and found that DENV-reactive MAbs were largely envelope specific and cross neutralizing. Many more MAbs neutralized DENV than reacted to envelope protein, emphasizing the significance of virion-dependent B cell epitopes and the limitations of envelope protein-based antibody screening. A majority of DENV-reactive MAbs, irrespective of neutralization potency, enhanced infection by antibody-dependent enhancement (ADE). Interestingly, even though DENV2 was the infecting serotype in all four patients, several MAbs from two patients neutralized DENV1 more potently than DENV2. Further, half of all type-specific neutralizing MAbs were also DENV1 biased in binding. Taken together, these findings are reminiscent of original antigenic sin (OAS), given that the patients had prior dengue virus exposures. These data describe the ongoing B cell response in secondary patients and may further our understanding of the impact of antibodies in dengue virus pathogenesis. © 2016, American Society for Microbiology.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84971449627&doi=10.1128%2fJVI.03203-15&partnerID=40&md5=600004ba65c907c8f4bc10d33fb0c0bb
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Young Adult
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Priyamvada L.. "B cell responses during secondary dengue virus infection are dominated by highly cross-reactive, memory-derived plasmablasts". ไม่ระบุหน่วยงาน. ม.ป.ป.

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B cell responses during secondary dengue virus infection are dominated by highly cross-reactive, memory-derived plasmablasts
Priyamvada L.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Characterization of human CD8 T cell responses in dengue virus-infected patients from India Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand Generation and characterization of cross neutralizing human monoclonal antibody against 4 serotypes of dengue virus without enhancing activity Identification of changes in dendritic cell subsets that correlate with disease severity in dengue infection Relationships between dengue virus infection in mosquito vector, (Aedes aegypti), dengue cases and weather conditions in Samut Sakhon Province, Thailand Microparticles provide a novel biomarker to predict severe clinical outcomes of dengue virus infection Potent neutralizing human monoclonal antibodies preferentially target mature dengue virus particles: Implication for novel strategy for dengue vaccine Adaptor protein 1A facilitates dengue virus replication Actin interacts with dengue virus 2 and 4 envelope proteins Biology of Zika virus infection in human skin cells
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