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CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN
Li Boxun Huang Guixiu - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN
ผู้แต่ง / หัวหน้าโครงการ (EN): Li Boxun Huang Guixiu
บทคัดย่อ (EN): In the last twenty years, Corynespora leaf fall disease (CLFD) has become one of the most serious leaf diseases, severely affecting the international rubber industry. It is currently responsible for 20-25% of the economic losses in natural rubber production. CLFD has been reported by the rubber producing countries in Asia, which account for 94% of the world’s natural rubber production. Identification of resistant clones is a major strategy in the management of CLFD in rubber. On screening the clones of rubber under natural conditions, we found that ‘IAN873’ was a resistant germplasm, and ‘PR107’ was susceptible to CLFD. In order to illustrate the resistant and susceptible response of the rubber tree (Hevea brasiliensis) infected with Corynespora cassiicala at a transcriptional level, gene expression profiles in leaves of rubber tree, inoculated with fungal spore suspension, were tested using cDNA-AFLP technology. Selective amplifications with 96 primer combinations allowed the visualization of 4,800 transcript-derived fragments (TDFs) in infected leaves, and 560 of them were found to be differentially expressed (306 induced and 254 repressed). 159 TDFs were cloned and sequenced, of which 116 were shown to have known functions and had predicted gene products by searching the GenBank database through BLAST. Based on the conclusion of the cDNA-AFLP analysis, four resistance genes were cloned by RT-PCR and PCR technique. There are a plant resistance NPR gene family member HbNPR1, a haloalcanoic acid dehalogenase super family member HbVSP2 and resistance gene analog members HbRGH2 and HbRGA-1. The expressed patterns of four representative TDFs were further confirmed by real-time PCR analysis. These differentially expressed genes may be involved in the processes of the anti/flu disease and our study confirms the effectiveness of isolation and cloning disease resistance genes.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): qRT-PCR
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Li Boxun Huang Guixiu. "CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN". ไม่ระบุหน่วยงาน. ม.ป.ป.

Li Boxun Huang Guixiu. "CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN
Li Boxun Huang Guixiu
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Differential Expression Analysis by cDNA-AFLP of Hevea brasiliensis after Inoculation with the Pathogen Corynespora cassiicola Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis) Diversity Analysis of Corynespora cassiicola Isolated from Indonesian Rubber Plantations Cloning and expression of HbACO2 of Hevea brasiliensis Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis) Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. Chloroplast DNA Microsatellite Differentiation in Cultivated and Wild Rubber Tree (Hevea brasiliensis) Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)
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