สืบค้นงานวิจัย
Localization of Peroxidase Enzyme in the Bark of Hevea brasiliensis
Gopika Gopal - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Localization of Peroxidase Enzyme in the Bark of Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Gopika Gopal
บทคัดย่อ (EN): Hevea brasiliensis, the Para rubber tree, is the prime source of natural rubber, exploited by means of controlled wounding called tapping to obtain latex from the tree trunk. It is a deciduous tropical tree which exhibits seasonal activity of tissues that leads to alter the anatomy of bark periodically. Anatomically, the bark of Hevea can be demarcated into inner soft bark and outer hard bark among which functionally active tissues likes sieve tubes, phloic rays, etc. are confined to the soft bark and those tissues in the hard bark region are less functional or shriveled. Localization of peroxidase enzyme with respect to seasons has been carried out in the bark of mature trees of Hevea brasiliensis using guaiacol and hydrogen peroxide as the substrate. Cell walls of sieve tubes including sieve plates, and cytoplasm of phloic rays, both in the soft and hard bark region of the bark showed positive indication for peroxidase activity with reddish brown coloration. Phloic rays exhibits seasonal variation for this enzyme while the activity is localized throughout the year in the sieve tubes. Sieve tubes differentiated recently from the derivatives of cambium also showed high enzyme activity. Companion cells are not giving any indication on peroxidase activity but it is localized in the intercellular spaces of axial parenchyma cells in the soft bark. The cell walls of the phloic rays remain passive for peroxidase activity throughout the year whereas the stained cytoplasm appears to be granulated and is in a state of streaming motion. Bark samples collected in the month of January and April showed deep coloration for phloic rays extending from the phellogen to the periphery of the cambial zone. In the month of June, the partially differentiated phloic rays in the cambial zone stained for peroxidase and subsequently differentiated ones are completely unstained. The phloic rays in the month of September remain unstained in the soft bark while it gave a brown coloration in the hard bark region. In the December samples, phloic rays are unstained throughout its extent in the bark. The deciduous nature of the tree is thus is thus evident for peroxidase activity too, which is observed in the cytoplasm of the phloic rays but not for sieve tubes.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): phellogen
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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